Chitosan is a polysaccharide which has found uses in many industries, including cosmetics, artificial skin and chitosan-based dressings, nutrition, contact lenses, and many others. The amount of deacetylation observed in the final chitosan product, also termed the degree of deacetylation, is often presented as a percentage (DDA%). This value is crucial to the physical and chemical properties of the chitosan, and while different assays exist to determine this value, NMR remains the industry standard, as demonstrated by its inclusion in the USP-NF monograph for chitosan testing. In this application note, we demonstrate equivalency to the current USP-NF monograph assay method, using benchtop quantitative nuclear magnetic resonance (qNMR) spectroscopy.