We recommend concentrations 0.15 M and higher because data, including accurate integrals, can be acquired in a typical 16 scans at this concentration.

When considering a 60 MHz NMR spectrometer, please review the questions posed below to access the effectiveness of the NMReady for your specific application(s).

What is the molecular weight range of the compounds that you work with?

As a rough guide, NMR at 60 MHz works well on compounds under 500 g/mol. This rule is generally true for compounds that are comprised predominantly by organic small molecules. There are two obvious exceptions to this:

  • Your molecules contain a large number of heteroatoms (Si, S, Br etc.) that increase the molecular weight without increasing the number of similar organic moieties (e.g., -CH, -CH2, or –CH3).

  • Your material is polymeric. Polymers are made of repeating units and typically afford broad NMR spectra by nature so the resolution is not as important in each spectrum.

What resolution is required to complete the analysis at hand?

It is important to note that the term ‘resolution’ refers to two separate measures. First, there is resolution in terms of chemical shift distribution. As heavy or complex molecules will have chemical shift overlap, this is the fundamental limitation to a low-field instrument.

The second type of resolution refers to the ‘J-couplings’ that can be resolved. The NMReady functions with a line width of <1.0 Hz, so it can resolve the majority of geminal and vicinal couplings. This is suitable for full structural elucidation of molecules with a certain molar mass range, such as those commonly synthesized in academic laboratories. The NMReady has suitable resolution for most reaction monitoring applications, polymer NMR and QA/QC applications.

What is the scale of the reactions you usually perform?

As a corollary, how much material is available for your samples?

If you perform large-scale reactions, are checking starting materials, or running spectra on demonstration compounds in an academic teaching environment then your application is typically not sample limited. Subsequently, we recommend that you make concentrated samples and run a relatively small number of scans. That way you can acquire the data you need in a timely fashion. If you are material and/or solubility limited, lower concentrations are more than acceptable, but it will require more time be committed to data acquisition so a sufficient number of scans can be run to meet your SNR target.

If you are using NMR quantitatively, one must determine what level of error is acceptable in the estimates of peak integrals and adjust the number of scans accordingly. More scans averaged will reduce the error in peak integral estimates. A convenient short cut is, in order to reduce uncertainties by a factor of two, the number of scans will need to be increased by a factor of four (scans and SNR are in a square relationship). In quantitative applications, it is also important to remember that nuclear spins in different chemical environments may have different relaxation times. In order for this not to introduce systematic errors in peak integral estimates, spins should be allowed to relax fully between scans (i.e., a longer interscan delay time may be required).

If you are unsure as to whether or not your application is amenable to the NMReady, please don’t hesitate to contact us for more information.